Coding

Part:BBa_K1547009:Design

Designed by: victor yuan   Group: iGEM14_Concordia   (2014-10-08)


Cas9 NLS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3823
    Illegal PstI site found at 244
    Illegal PstI site found at 2212
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
    Illegal PstI site found at 3962
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3823
    Illegal PstI site found at 244
    Illegal PstI site found at 2212
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
    Illegal PstI site found at 3962
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3823
    Illegal BglII site found at 251
    Illegal BglII site found at 1188
    Illegal BamHI site found at 2006
    Illegal XhoI site found at 3544
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3823
    Illegal PstI site found at 244
    Illegal PstI site found at 2212
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
    Illegal PstI site found at 3962
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3823
    Illegal PstI site found at 244
    Illegal PstI site found at 2212
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
    Illegal PstI site found at 3962
    Illegal NgoMIV site found at 2740
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2886
    Illegal BsaI site found at 3740
    Illegal BsaI.rc site found at 1411


Design Notes

Large - required tampering with PCR conditions.


Source

TEF-414 strain

References